Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope

نویسندگان

چکیده

Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization a diffractive optical element producing several light sheets along an inclined tube for applications in flow-driven imaging. The element, which is based on multi-focal Fresnel zone plate linear grating, generates combination refractive cylindrical lens five thin equal intensity.

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ژورنال

عنوان ژورنال: Epj Web of Conferences

سال: 2022

ISSN: ['2101-6275', '2100-014X']

DOI: https://doi.org/10.1051/epjconf/202226604004